#!/usr/bin/env python
# This code is part of the Biopython distribution and governed by its
# license.  Please see the LICENSE file that should have been included
# as part of this package.
#
"""Run and process output from the Wise2 package tool dnal.

Bio.Wise contains modules for running and processing the output of
some of the models in the Wise2 package by Ewan Birney available from:
ftp://ftp.ebi.ac.uk/pub/software/unix/wise2/
http://www.ebi.ac.uk/Wise2/

Bio.Wise.psw is for protein Smith-Waterman alignments
Bio.Wise.dnal is for Smith-Waterman DNA alignments
"""

from __future__ import print_function

import re

# Importing with leading underscore as not intended to be exposed
from Bio._py3k import getoutput as _getoutput
from Bio._py3k import zip
from Bio._py3k import map

from Bio import Wise


_SCORE_MATCH = 4
_SCORE_MISMATCH = -1
_SCORE_GAP_START = -5
_SCORE_GAP_EXTENSION = -1

_CMDLINE_DNAL = ["dnal", "-alb", "-nopretty"]


def _build_dnal_cmdline(match, mismatch, gap, extension):
    res = _CMDLINE_DNAL[:]
    res.extend(["-match", str(match)])
    res.extend(["-mis", str(mismatch)])
    res.extend(["-gap", str(-gap)])  # negative: convert score to penalty
    res.extend(["-ext", str(-extension)])  # negative: convert score to penalty

    return res


_CMDLINE_FGREP_COUNT = "fgrep -c '%s' %s"


def _fgrep_count(pattern, file):
    return int(_getoutput(_CMDLINE_FGREP_COUNT % (pattern, file)))


_re_alb_line2coords = re.compile(r"^\[([^:]+):[^\[]+\[([^:]+):")


def _alb_line2coords(line):
    return tuple([int(coord) + 1  # one-based -> zero-based
                  for coord
                  in _re_alb_line2coords.match(line).groups()])


def _get_coords(filename):
    alb = open(filename)

    start_line = None
    end_line = None

    for line in alb:
        if line.startswith("["):
            if not start_line:
                start_line = line  # rstrip not needed
            else:
                end_line = line

    if end_line is None:  # sequence is too short
        return [(0, 0), (0, 0)]

    return list(zip(*map(_alb_line2coords, [start_line, end_line])))  # returns [(start0, end0), (start1, end1)]


class Statistics(object):
    """Calculate statistics from an ALB report."""

    def __init__(self, filename, match, mismatch, gap, extension):
        self.matches = _fgrep_count('"SEQUENCE" %s' % match, filename)
        self.mismatches = _fgrep_count('"SEQUENCE" %s' % mismatch, filename)
        self.gaps = _fgrep_count('"INSERT" %s' % gap, filename)

        if gap == extension:
            self.extensions = 0
        else:
            self.extensions = _fgrep_count('"INSERT" %s' % extension, filename)

        self.score = (match * self.matches +
                      mismatch * self.mismatches +
                      gap * self.gaps +
                      extension * self.extensions)

        if self.matches or self.mismatches or self.gaps or self.extensions:
            self.coords = _get_coords(filename)
        else:
            self.coords = [(0, 0), (0, 0)]

    def identity_fraction(self):
        return self.matches / (self.matches + self.mismatches)

    header = "identity_fraction\tmatches\tmismatches\tgaps\textensions"

    def __str__(self):
        return "\t".join(str(x) for x in (self.identity_fraction(),
                                          self.matches, self.mismatches,
                                          self.gaps, self.extensions))


def align(pair, match=_SCORE_MATCH, mismatch=_SCORE_MISMATCH, gap=_SCORE_GAP_START, extension=_SCORE_GAP_EXTENSION, **keywds):
    cmdline = _build_dnal_cmdline(match, mismatch, gap, extension)
    temp_file = Wise.align(cmdline, pair, **keywds)
    try:
        return Statistics(temp_file.name, match, mismatch, gap, extension)
    except AttributeError:
        try:
            keywds['dry_run']
            return None
        except KeyError:
            raise


def main():
    import sys
    stats = align(sys.argv[1:3])
    print("\n".join("%s: %s" % (attr, getattr(stats, attr))
                    for attr in ("matches", "mismatches", "gaps", "extensions")))
    print("identity_fraction: %s" % stats.identity_fraction())
    print("coords: %s" % stats.coords)


def _test(*args, **keywds):
    import doctest
    import sys
    doctest.testmod(sys.modules[__name__], *args, **keywds)


if __name__ == "__main__":
    if __debug__:
        _test()
    main()
